Incomplete Freund’s adjuvant is usually prepared with a mixture of 2 parts of liquid paraffin and one part of lanolin, but the ratio may be 1-5:1, according to the need. An incomplete adjuvant can be transformed into a complete Freund’s adjuvant with the final concentration of 2-20 mg/ml of BCG or killed Mycobacterium tuberculosis. Usually, during the first injection, emulsification is used with 1/2 volume of FCA and antigen, and no adjuvant is used for the subsequent second and third inoculation. The antigen volume can be increased by 10 to 20 times without adding an adjuvant. https://www.changyubio.com/freunds-adjuvant/

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